Anti-Apo E antibody
An antibody raised against apolipoprotein E (ApoE); a lipotransport protein that combines with lipids for transport to maintain lipid homeostasis.
Catalogue number crb2005648 Antibody Anti-Apo E antibody Antigen Peptide KLH conjugated synthetic peptide crb1200857e Protein ID P08226 Mouse Apoe, P02650 Rat Apoe Aliases Apolipoprotein E, Apo-E, Apoe Cross-Reactivity Mouse, Rat Target Protein Species Mouse, Rat Host Species Rabbit Antibody Type Polyclonal Concentration 1mg/ml Glycine (R1G), 1mg/ml Glycine (R2G) Target Apo E Storage Stabilisers The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. Storage The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. References
Flowers et al., (2020). APOE in the normal brain. Neurobiol. Dis. 136: 104724. DOI: https://doi.org/10.1016/j.nbd.2019.104724.
Holtzman et al., (2012). Apolipoprotein E and apolipoprotein E receptors: normal biology and roles in Alzheimer disease. Cold Spring Harb. Perspect. 2(3): a006312. DOI: https://doi.org/10.1101/cshperspect.a006312.
Liu et al., (2013). Apolipoprotein E and Alzheimer disease: risk, mechanisms and therapy. Nat. Rev. Neurol. 9(2): 106. DOI: https://doi.org/10.1038/nrneurol.2012.263.
ApoE is part of the lipoprotein and involved in its production, conversion, and clearance. ApoE associates with lipid molecules such as chylomicrons, very low-density lipoproteins (VLDL), intermediate density lipoproteins (IDL) and high-density lipoproteins (HDL). ApoE is the main carrier of cholesterol in humans, it functions in the transport of HDLs containing cholesterol from peripheral tissues to the liver and mediates their uptake by hepatocyte for clearance. ApoE can mediate clearance of any of the lipid molecules mentioned by hepatocytes but has a preferential binding for HDLs. In addition to this role, ApoE also has heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, which aids entry of ApoE-containing lipoproteins by receptor mediated uptake.
ApoE also plays a role in lipid transport in the central nervous system aiding neural cell survival. APOE has 3 isoforms, of which, APOE ε4 has been characterised as a major risk factor for Alzheimer disease and for an increased risk of cerebral amyloid angiopathy. The formation of amyloid β aggregates leading to plaques has been well documented to be linked to the functionality/isoform of ApoE present. Finally, ApoE4 has been linked to hyperlipidaemia and hypercholesterolemia. These conditions lead to atherosclerosis, coronary heart disease and stroke. This antibody could be a vital tool to further the research into a protein in numerous fields.