Antibody raised against ANO1, a protein used in transepithelial anion transport and smooth muscle contraction.
Catalogue number crb2005665 Antibody Anti-ANO1 antibody Antigen Peptide KLH conjugated synthetic peptide crb1200874e Protein ID Q5XXA6 ANO1 Aliases Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2 Host Species Rabbit Antibody Type Polyclonal Concentration 4.0mg/ml Glycine (R2G), 2.5mg/ml Glycine (R1G) Target ANO1 Storage Stabilisers The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. Family Anoctamin family Storage The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. References
Dulin, N.O. (2020). Calcium-Activated Chloride Channel ANO1/TMEM16A: Regulation of Expression and Signaling. Frontiers in Physiology, 11(590262).
Singh, R.D., et al. (2014). Ano1, a Ca2+-activated Cl−channel, coordinates contractility in mouse intestine by Ca2+transient coordination between interstitial cells of Cajal. The Journal of Physiology, 592(18), pp.4051–4068.
Stanich, J.E., et al. (2011). Ano1 as a regulator of proliferation. American Journal of Physiology-Gastrointestinal and Liver Physiology, 301(6), pp.G1044–G1051.
ANO1 is a calcium-activated chlorine channel protein, expressed in the epithelia, with this expression in the Interstitial Cells of Cajal (ICC) being responsible for the slow wave peristalsis in the gastrointestinal tract. The ICC are the pacemaker cells for the gut, involved in the propagation of the slow gastrointestinal wave, required to move ingested material through the gut. Blocking or knockout of ANO1 is reported to result in the loss of organised smooth muscle contraction responsible for peristalsis, suggesting ANO1 is essential for the normal function of gastrointestinal physiology. However, ANO1 is found in all ICC cells, including those not responsible for pacemaker function, implying there are unknown additional functions.
In addition to the function within the gut, ANO1 is expressed in several epithelia, controlling several cell functions including proliferation, survival, migration and secretion. ANO1 was initially discovered due to high expression of the channel proteins in gastrointestinal stromal tumours (GISTs), and was previously known as DOG1 (discovered on GISTs).