An antibody raised against Shugoshin 1 (Sgo1). Sgo1 aids in timely chromosome segregation during mitosis and meiosis by protecting the interaction of cohesin to the centromere.
Images provided courtesy of Debojit Saha
|Antigen Peptide||KLH conjugated synthetic peptide crb1200545e|
|Target Protein Species||Mouse|
|Concentration||1 mg/ml Glycine eluate|
|Target||mouse Shugoshin 1|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Disease Area||Cancer, DNA damage|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Xu et al., (2009). Structure and function of the PP2A-shugoshin interaction. Mol cell, 35(4): 426. DOI: 10.1016/j.molcel.2009.06.031.
Yin et al., (2008). Shugoshin1 may play important roles in separation of homologous chromosomes and sister chromatids during mouse oocyte meiosis. PloS one, 3(10): e3516. PMID: 18949044.
Timely chromosome segregation during mitosis and meiosis is orchestrated by separase activity cleaving the multi-subunit cohesin complex from the centromere. Sister chromosomes remain attached at the centromere till after meiosis I anaphase due to shugoshins protecting cohesin from separase activity. Recent work shows shugoshin’s role is to interact with PP2A and maintaining cohesin in a dephosphorylated state thus protecting the centromeric RecB protein from separase activity.
In mammals, there are 2 known sugoshins paralogues, Sgo1 and Sgo2. Sgo1 appears to play the primary role in cohesin protection during mitosis when most cohesin complex dissociates from the chromosome arms. Whereas Sgo2 aids centromeric cohesion during meiosis. Reduced levels of Sgo1 have been associated with colorectal cancers due to the increased chromosomal instability leading to tumourigenesis. Defective Sgo1 has also been linked to aneuploidy. This KLH conjugated Sgo1 antibody is the ideal research tool to investigate the functions of shugoshins as there is still much to uncover.