ThioLinker-DBCO is a water-soluble bis-alkylating reagent that allows for the site-specific addition of a DBCO group onto antibodies, without disrupting either their tertiary structure or their biological activity.
Catalogue number crb7108358 Molecular Weight 1140.39 Chemical Composition C59H69N3O14S3 Appearance White to grey amorphous solid Solubility Water, DMSO, DMF, DCM, THF, Chloroform Chemistry DBCO Purity >95% (HPLC) cas N/A Storage -20C Manufacturer Product Number 1096
ThioLinker-DBCO is a water-soluble, bis-alkylating labelling reagent that enables the site-specific incorporation of DBCO moiety onto antibodies to form well-defined conjugates. This approach involves a disulfide reduction to release the two cysteine thiols followed by the re-forming the disulfide by bis-alkylation, via a three-carbon bridge to which DBCO moiety is covalently attached. During this process, irreversible denaturation of the protein, which would disrupt either their tertiary structure or their biological activity, does not occur. The covalent, site-specific conjugation of PEG to a polyhistidine tag (His-tag) on a protein with bis-alkylating reagents was also reported in literature.