DBCO-PEG4-Maleimide is a sulfhydryl-reactive labelling reagent with a hydrophilic PEG4 spacer arm.
Catalogue number crb7108360 Molecular Weight 674.74 Chemical Composition C36H42N4O9 Appearance Yellow to slightly orange oil Solubility DMSO, DMF, THF, Acetonitrile, Dichloromethane Chemistry Maleimide Purity >95% (HPLC) cas 1480516-75-3 Storage -20C Manufacturer Product Number A108P
DBCO-PEG4-Maleimide allows for simple combination of a DBCO group and thiol-containing molecules, such as cysteine-containing peptides. The maleimide group reacts with sulfhydryl groups at pH 6.5 – 7.5 to form stable thioether bonds. Its long length enhances accessibility of the azide-reactive DBCO and reactive thiols, which may be buried. The polyethylene glycol (PEG) spacer arm is hydrophilic and improves solubility in aqueous buffers.