DBCO-PEG4-Gly-Gly-Gly is a water-soluble substrate with extended PEG4 linker for the sortase-mediated labelling of proteins.
Catalogue number crb7108373 Molecular Weight 845.92 Chemical Composition C38H51N7O13S Appearance Grey amorphous solid Solubility Water, DMSO, DMF Chemistry DBCO Purity >95% (HPLC) Storage -20C Manufacturer Product Number 1552
DBCO-Gly-Gly-Gly is a water-soluble substrate for the sortase-mediated labelling of proteins (or “Sortagging”). Sortase catalyses a transpeptidase reaction (between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine) and has also been used in peptide synthesis to cyclize peptides (to create macrocyclic peptides, glycopeptides & protein−protein conjugates). Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins, site-specific protein-lipid conjugates, and constructing peptides & glycosylphosphatidylinositol chimeras.