Anti-RAF1 antibody
Description
Application Data
Description
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Recognises human Raf1 protein in Western blots and immunohistochemistry with a strong signal and low background noise
Application Data
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| Catalogue number | crb2005145 |
|---|---|
| Antibody | Anti-RAF1 antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb1200405e |
| Protein ID | UniProtKB - P04049 |
| Aliases | Raf-1 Proto-Oncogene, Serine/Threonine Kinase, Oncogene RAF1, CRAF, C-Raf |
| Cross-Reactivity | Human |
| Target Protein Species | Human |
| Host Species | Rabbit |
| Antibody Type | Polyclonal |
| Concentration | 1mg/ml |
| Validation | 1:100 (WB), 1:1000 (ELISA) |
| Target | RAF1 |
| Storage Stabilisers | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| Family | TKL Ser/Thr protein kinase family, RAF subfamily |
| Super Family | Tyrosine protein kinase family |
| Disease Area | Signal transduction |
| Storage | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| References | Buscaino, A., White, S., Houston, D., Lejeune, E., Simmer, F., de Lima Alves, F., Diyora, P., Urano, T., Bayne, E., Rappsilber, J. and Allshire, R. (2012). Raf1 Is a DCAF for the Rik1 DDB1-Like Protein and Has Separable Roles in siRNA Generation and Chromatin Modification. PLoS Genetics, 8(2), e1002499. PMID: 22319459 |
Raf1 forms part of the histone H3K9 methyltransferase complex CLRC, along with Clr4, Raf2, Cul4, and Rik1 and is required for heterochromatin formation. CLRC mediates H3K9 methylation, siRNA production and also displays E3-ubiquitin ligase activity in vitro.
Raf1 is a DCAF (DDB1 and CUL4 associated factor) for CLRC. DCAFs act as substrate receptors for E3 ligases and may couple ubiquitination with histone methylation. Raf1 plays an essential role in coupling H3K9 methylation and siRNA production.