Anti-RAF1 antibody

  • Description

  • Application Data

Description

Recognises human Raf1 protein in Western blots and immunohistochemistry with a strong signal and low background noise

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Application Data

Catalogue number crb2005145
Antibody Anti-RAF1 antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200405e
Protein ID UniProtKB - P04049
Aliases Raf-1 Proto-Oncogene, Serine/Threonine Kinase, Oncogene RAF1, CRAF, C-Raf
Cross-Reactivity Human
Target Protein Species Human
Host Species Rabbit
Antibody Type Polyclonal
Concentration 1mg/ml
Validation 1:100 (WB), 1:1000 (ELISA)
Target RAF1
Storage Stabilisers This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.
Family TKL Ser/Thr protein kinase family, RAF subfamily
Super Family Tyrosine protein kinase family
Disease Area Signal transduction
Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.
References

Buscaino, A., White, S., Houston, D., Lejeune, E., Simmer, F., de Lima Alves, F., Diyora, P., Urano, T., Bayne, E., Rappsilber, J. and Allshire, R. (2012). Raf1 Is a DCAF for the Rik1 DDB1-Like Protein and Has Separable Roles in siRNA Generation and Chromatin Modification. PLoS Genetics, 8(2), e1002499. PMID: 22319459

Data Sheet Data Sheet Material Safety Data Sheet (MSDS)

Raf1 forms part of the histone H3K9 methyltransferase complex CLRC, along with Clr4, Raf2, Cul4, and Rik1 and is required for heterochromatin formation. CLRC mediates H3K9 methylation, siRNA production and also displays E3-ubiquitin ligase activity in vitro.

Raf1 is a DCAF (DDB1 and CUL4 associated factor) for CLRC. DCAFs act as substrate receptors for E3 ligases and may couple ubiquitination with histone methylation. Raf1 plays an essential role in coupling H3K9 methylation and siRNA production.

Anti-RAF1 antibody