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Detection and characterisation of nduf11a from (RNAi)-treated worms.
A reduction of NDUF-11 protein in mitochondria after RNAi is detectable using the anti-nduf11a antibody. A total of 50 µg of isolated mitochondria is loaded per lane. Treatment with RNAi significant reduces detectable nduf11.

Control: C. elegans mitochondria anti-nduf11a antibody detects Nduf11a.
nduf-11: C. elegans mitochondria anti-nduf11a antibody detects a significant reduction in Nduf11a after RNAi treatment.
ATP5 is used as a loading control.
Primary: Anti-Nduf11a antibody (1: 500; 5% milk in TBS-T).
Secondary: Anti-rabbit conjugated to HRP.