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SUMO conjugation to FLS2 is flg22 dependant and required for innate immunity in Arabidopsis. FLS2 is rapidly SUMOylated after flg22 treatment. Immunoprecipitation (IP: αGFP) was carried out with anti-GFP beads from total protein derived from two independent transgenic lines expressing proFLS2::FLS2-GFP (fls2) treated either with MgCl2 or 1 µM flg22 for 10 min. Immunoblots were probed with anti-GFP (IB: αGFP) or anti-SUMO1/2 (IB: αSUMO1) antibodies. Col-0 was used as a negative control.