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Western blot analysis of HEK293T cell lysates with HAND-1 overexpression.

Lane 1: HEK293T cell lysates transiently transfected with TrueORF pCMV vector only (20µg)

Lane 2: HEK293T cell lysates transiently transfected with TrueORF pCMV-HAND-1 plasmid (20µg)

Secondary: Rabbit anti-sheep IgG 1:2000

* HAND-1 dimer and trimer at ~50kDa and ~75kDa